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KMID : 0624620120450030183
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BMB Reports
2012 Volume.45 No. 3 p.183 ~ p.188
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Human Intersectin 2 (ITSN2) binds to Eps8 protein and enhances its degradation
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Ding Xiaofeng
Yang Zijian
Zhou Fangliang
Hu Xiang
Zhou Chang
Luo Chang
He Zhicheng
Liu Qian
Li Hong
Yan Feng
Wang Fangmei
Xiang Shuanglin
Zhang Jian
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Abstract
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Participates in actin remodeling through Rac and receptor endocytosis via Rab5. Here, we used yeast two-hybrid system with Eps8 as bait to screen a human brain cDNA library. ITSN2 was identified as the novel binding factor of Eps8. The interaction between ITSN2 and Eps8 was demonstrated by the in vivo co-immunoprecipitation and colocalization assays and the in vitro GST pull-down assays. Furthermore, we mapped the interaction domains to the region between amino acids 260-306 of Eps8 and the coiled-coil domain of ITSN2. In addition, protein stability assays and immunofluorescence analysis showed ITSN2 overexpression induced the degradation of Eps8 proteins, which was markedly alleviated with the lysosome inhibitor NH4Cl treatment. Taken together, our results suggested ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins.
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KEYWORD
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Eps8, Interaction, ITSN2, Lysosome pathway, Protein degradation
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